Investigations into the Nature, Causation, and Prevention of Texas or Southern Cattle Fever Page: 43
301 p. : ill. (some col.) ; 24 cm.View a full description of this book.
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THE NATURE OF TEXAS CATTLE FEVER.
same as those used in the important study of the micro-organism or
blood-parasite of this disease.
The blood was examined in the fresh and in the dried condition. The
drop of blood as it oozed from the incision was received at once on a
flamed platinum loop soldered into a glass rod like the ordinary bac-
teriological loops used for inoculating, etc. The platinum loop is simply
brought in contact with the blood, and the drop placed on a clean glass
slide and immediately covered with a cover glass and sealed with paraf-
fin if the preparation is to be kept under observation for some time.
The sealing is best done with a camel's hair brush dipped into melted
paraffin. It is always desirable to have only a single layer of cor-
puscles in the preparation. To insure this there should be no speck of
dust on slide or cover, and the quantity of blood taken must be small.
This can be regulated by adjusting the size of the platinum loop. In
the usual method of touching the drop of blood with the cover di-
rectly the quantity of blood can not be limited, and many preparations
are subsequently found to contain too many corpuscles.
The preparation of dried blood requires much care. Without going
into an extended discussion of the relative merits of different methods
we give the one found most satisfactory and adopted in these investi-
gations. It is most readily understood by referring to the annexed
figure:
/ x
A B
ir. 2.-Method of preparing dry blood films on cover glasses.
In A, a cover glass cc is held by a pair of forceps b and has on its
upper surface a drop of blood a placed there by a platinum loop. A
second cover glass or "scraper" held by forceps is resting on the first
with one edge and is held at an angle of 150 to 200 to it. As shown in
B the upper cover is drawn over the lower, and as its edge sweeps over
it it spreads out the drop of blood in a thin layer. In rapid work the
forceps may be dispensed with. The lower cover is held between thumb
and forefinger of the left hand at cc and steadied below with the tip of
the middle finger, and the upper is firmly seized between thumb and
forefinger of the right where the blades of the forceps would rest.43
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Smith, Theobold & Kilborne, Fred Lucius. Investigations into the Nature, Causation, and Prevention of Texas or Southern Cattle Fever, book, 1893; Washington D.C.. (https://texashistory.unt.edu/ark:/67531/metapth143538/m1/43/: accessed July 16, 2024), University of North Texas Libraries, The Portal to Texas History, https://texashistory.unt.edu; crediting University of Texas Health Science Center Libraries.